Whole conversion of agro-industrial wastes rich in galactose-based carbohydrates into lipid using oleaginous yeast Aureobasidium namibiae

Abstract Background Raw materials composed of easily assimilated monosaccharides have been employed as carbon source for production microbial lipids. Nevertheless, agro-industrial wastes rich in galactose-based carbohydrates not introduced feedstocks oleaginous yeasts. Results In this study, Aureobasidium namibiae A12 was found to efficiently accumulate lipid from soy molasses and whey powder containing carbohydrates, with productions 5.30 g/L 5.23 g/L, respectively. Over 80% the fatty acids C 16:0 , 18:0 18:1 18:2 . All kinds single sugar components two byproducts were readily converted into lipids, yields ranging between 0.116 g/g 0.138 g/g. Three α-galactosidases five β-galactosidases strain cloned analyzed. Changes transcriptional levels indicated GalB GalC key α-galactosidases, GalG β-galactosidase. 10 L fermentor, SM WP achieved 6.45 6.13 β-galactosidase responsible lactose hydrolysis; sucrase α-galactosidase both contributed efficient hydrolysis raffinose stachyose a cooperation manner. Conclusions This is new way produce lipids raw carbohydrates. finding revealed significance direct first time facilitated understanding utilization manufacture or other chemicals bioprocess. Graphic abstract